model 620 video densitometer Search Results


digital scanning densitometer  (Bio-Rad)
88
Bio-Rad digital scanning densitometer
Digital Scanning Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer densitometry  (Bio-Rad)
96
Bio-Rad computer densitometry
Computer Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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densitometry  (Bio-Rad)
93
Bio-Rad densitometry
Figure 1. Chromatographic purification of E. coli oh8Gua endonuclease. After removal of nucleic acids with streptomycin, crude extract was separated by first phenyl HPLC (A), DEAE HPLC (B), gel filtration chromatography (C), second phenyl HPLC (D) and heparin affinity HPLC (E). For activity assay, 5 or 10 µl of each fraction was incubated with 5’ end-labeled substrate DNA containing oh8Gua. DNA nick at the site of oh8Gua was visualized with sequencing gel electrophoresis and autoradiography. For collection of active fractions, percentage of cleavage was determined by <t>densitometry.</t> Solid line, dashed line and dotted solid line indicate absorbance at 280 nm (protein profile; arbitrary unit except gel filtration chromatography), salt concentration and activity (% of nick), respectively.
Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/densitometry/product/Bio-Rad
Average 93 stars, based on 1 article reviews
densitometry - by Bioz Stars, 2026-05
93/100 stars
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Image Search Results


Figure 1. Chromatographic purification of E. coli oh8Gua endonuclease. After removal of nucleic acids with streptomycin, crude extract was separated by first phenyl HPLC (A), DEAE HPLC (B), gel filtration chromatography (C), second phenyl HPLC (D) and heparin affinity HPLC (E). For activity assay, 5 or 10 µl of each fraction was incubated with 5’ end-labeled substrate DNA containing oh8Gua. DNA nick at the site of oh8Gua was visualized with sequencing gel electrophoresis and autoradiography. For collection of active fractions, percentage of cleavage was determined by densitometry. Solid line, dashed line and dotted solid line indicate absorbance at 280 nm (protein profile; arbitrary unit except gel filtration chromatography), salt concentration and activity (% of nick), respectively.

Journal: Experimental & molecular medicine

Article Title: Identification of Escherichia coli 8-oxoguanine endonuclease.

doi: 10.1038/emm.2000.26

Figure Lengend Snippet: Figure 1. Chromatographic purification of E. coli oh8Gua endonuclease. After removal of nucleic acids with streptomycin, crude extract was separated by first phenyl HPLC (A), DEAE HPLC (B), gel filtration chromatography (C), second phenyl HPLC (D) and heparin affinity HPLC (E). For activity assay, 5 or 10 µl of each fraction was incubated with 5’ end-labeled substrate DNA containing oh8Gua. DNA nick at the site of oh8Gua was visualized with sequencing gel electrophoresis and autoradiography. For collection of active fractions, percentage of cleavage was determined by densitometry. Solid line, dashed line and dotted solid line indicate absorbance at 280 nm (protein profile; arbitrary unit except gel filtration chromatography), salt concentration and activity (% of nick), respectively.

Article Snippet: To determine the collection of active fractions, % of DNA breakage was estimated by densitometry (Model 620, BioRad, USA).

Techniques: Purification, Filtration, Chromatography, Activity Assay, Incubation, Labeling, Sequencing, Nucleic Acid Electrophoresis, Autoradiography, Concentration Assay